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Promega improm ii rt kit
Improm Ii Rt Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/improm+ii+rt+system+kit/improm+ii+rt+kit/10__1113_slash_jp286266-112-12-16
Average 90 stars, based on 1 article reviews
improm ii rt kit - by Bioz Stars, 2026-10
90/100 stars

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Reverse Transcription:

Article Title: Histone deacetylase inhibitors impair antibacterial defenses of macrophages.
Article Snippet: RNA was isolated using the RNeasy kit (Qiagen). .. Reverse transcription was performed using the ImProm II RT System kit (Promega). .. Quantitative real-time polymerase chain reaction (PCR) was performed with a 7500 Fast Real-Time PCR System using the Power SYBR Green PCR Master Mix (Applied Biosystems) and primer pairs (Supplementary Table 1) as previously described [19].

Article Title: Estradiol and Progesterone Strongly Inhibit the Innate Immune Response of Mononuclear Cells in Newborns
Article Snippet: Total RNA was isolated from CBMCs and PBMCs using an RNeasy kit (Qiagen, Hombrechtikon, Switzerland). .. Reverse transcription of 1 g of RNA was performed using an ImProm II RT system kit (Promega, Dübendorf, Switzerland). .. Real-time PCR was performed with a 7500 Fast real-time PCR system (Applied Biosystems, Rotkreuz, Switzerland) using the Power SYBR green PCR master mix (Applied Biosystems) as described previously (29).

Article Title: Estradiol and Progesterone Strongly Inhibit the Innate Immune Response of Mononuclear Cells in Newborns
Article Snippet: Total RNA was isolated from CBMCs and PBMCs using an RNeasy kit (Qiagen, Hombrechtikon, Switzerland). .. Reverse transcription of 1 μg of RNA was performed using an ImProm II RT system kit (Promega, Dübendorf, Switzerland). .. Real-time PCR was performed with a 7500 Fast real-time PCR system (Applied Biosystems, Rotkreuz, Switzerland) using the Power SYBR green PCR master mix (Applied Biosystems) as described previously ( 29 ).

Article Title: Deletion of the Viral Anti-Apoptotic Gene F1L in the HIV/AIDS Vaccine Candidate MVA-C Enhances Immune Responses against HIV-1 Antigens
Article Snippet: Total RNA was isolated using the RNeasy kit (Qiagen) from murine BMDMs or BMDCs mock-infected or infected at 1 or 5 PFU/cell with MVA-C or MVA-C-ΔF1L for 6 hours. .. Reverse transcription of 100 ng to 500 ng of RNA was performed using the ImProm II RT System kit (Promega). .. Quantitative PCR was performed with a 7500 Fast Real-Time PCR System (Applied Biosystems) using the Power SYBR Green PCR Master Mix (Applied Biosystems).

Article Title: A Candidate HIV/AIDS Vaccine (MVA-B) Lacking Vaccinia Virus Gene C6L Enhances Memory HIV-1-Specific T-Cell Responses
Article Snippet: Total RNA was isolated from THP-1 cells and moDCs infected with MVA, MVA-B and MVA-B ΔC6L, using the RNeasy kit (Qiagen, Hombrechtikon, Switzerland). .. Reverse transcription of 100 ng to 500 ng of RNA was performed using the ImProm II RT System kit (Promega). .. Quantitative PCR was performed with a 7500 Fast Real-Time PCR System (Applied Biosystems, Rotkreuz, Switzerland) using the Power SYBR Green PCR Master Mix (Applied Biosystems), as previously described .

Article Title: Interleukin-1- and Type I Interferon-Dependent Enhanced Immunogenicity of an NYVAC-HIV-1 Env-Gag-Pol-Nef Vaccine Vector with Dual Deletions of Type I and Type II Interferon-Binding Proteins
Article Snippet: 192 Total RNA was isolated from THP-1 cell lines using the RNeasy kit (Qiagen, 193 Hombrechtikon, Switzerland). .. Reverse transcription of 1 g of RNA was performed 194 using the ImProm II RT System kit (Promega, Dübendorf, Switzerland). .. Quantitative 195 PCR was performed with a 7500 Fast Real-Time PCR System (Applied Biosystems, 196 Rotkreuz, Switzerland) using the Power SYBR Green PCR Master Mix (Applied 197 Biosystems).

Article Title: Innate Immune Sensing of Modified Vaccinia Virus Ankara (MVA) Is Mediated by TLR2-TLR6, MDA-5 and the NALP3 Inflammasome
Article Snippet: Total RNA was isolated from THP-1 cell lines, human monocytes/macrophages, peritoneal cells and splenocytes using the RNeasy kit (Qiagen, Hombrechtikon, Switzerland). .. Reverse transcription of 1 μg of RNA was performed using the ImProm II RT System kit (Promega, Dübendorf, Switzerland). .. Quantitative PCR was performed with a 7500 Fast Real-Time PCR System (Applied Biosystems, Rotkreuz, Switzerland) using the Power SYBR Green PCR Master Mix (Applied Biosystems) and primer pairs listed in .



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